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THE ADHESION OF ODONTOBLAST TO TYPE I COLLAGEN
¾È¸í±â, Á¤¼ºÈñ, ±è½Å,
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¾È¸í±â ( Ahn Myung-Ki ) - ºÎ»ê´ëÇб³ Ä¡ÀÇÇÐÀü¹®´ëÇпø ¼Ò¾ÆÄ¡°úÇб³½Ç
Á¤¼ºÈñ ( Jeong Sung-Hee ) - ºÎ»ê´ëÇб³ Ä¡ÀÇÇÐÀü¹®´ëÇпø ¼Ò¾ÆÄ¡°úÇб³½Ç
±è½Å ( Kim Shin ) - ºÎ»ê´ëÇб³ Ä¡ÀÇÇÐÀü¹®´ëÇпø ¼Ò¾ÆÄ¡°úÇб³½Ç
KMID : 0358920100370030308
Abstract
»ó¾Æ¸ð¼¼Æ÷´Â ºÎÂøºÐÀÚµéÀ» ÀÌ¿ëÇÏ¿© ±âÁú¿¡ ºÎÂøÇÏ´Â ¼¼Æ÷À̸ç, ÀÎÅױ׸°°ú °°Àº ºÎÂøºÐÀÚµéÀÌ ÀÏ·ÃÀÇ ¼¼Æ÷¿Í ¼¼Æ÷¿Ü±âÁúÀ» ÀÎÁöÇÏ´Â ½ÅÈ£Àü´ÞºÐÀÚ·Î ¾Ë·ÁÁ® ÀÖ´Ù. º» ¿¬±¸ÀÇ ¸ñÀûÀº »ó¾Æ¸ð¼¼Æ÷(MDPC-23 ¼¼Æ÷)¿Í IÇü ¾Æ±³Áú°úÀÇ »óÈ£ÀÛ¿ë°ú TGF-°ú TNF-°¡ ¼¼Æ÷ºÎÂøºÐÀÚÀÇ ¹ßÇö¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» ¾Ë¾Æº¸±â À§ÇØ ½ÃÇàÇÏ¿´´Ù. º» ¿¬±¸¿¡¼ MDPC-23 ¼¼Æ÷´Â ³óµµÀÇÁ¸ÀûÀ¸·Î IÇü ¾Æ±³Áú¿¡ ºÎÂøÇßÀ¸¸ç, ¸é¿ªÇü±¤¿°»ö¹ý¿¡¼ MDPC-23 ¼¼Æ÷°¡ ¾Æ±³Áú¿¡ ºÎÂøÇÒ ¶§, ±¹¼ÒºÎÂøÁ¡¿¡¼ ÀÎÅױ׸° , , CD44, FAK ±×¸®°í paxillinÀÇ ¹ßÇö¾ç»óÀ» °üÂûÇÒ ¼ö ÀÖ¾ú´Ù. ½ÎÀÌÅäÄ«ÀÎ TGF-Àº MDPC-23 ¼¼Æ÷ÀÇ ¾Æ±³Áú¿¡ ´ëÇÑ ºÎÂø¼º ¹× ÀÎÅױ׸° , ¿Í chondroitin sulfateÀÇ ¹ßÇöÀ» Áõ°¡½ÃÄ×À¸¸ç, RT-PCRÀÇ °á°ú¿¡¼´Â ÀÎÅױ׸° ÀÇ mRNAÀÇ ¾çÀÌ TGF-¿¡ ÀÇÇؼ Áõ°¡µÇ¾úÀ½À» È®ÀÎÇÏ¿´´Ù. °á·ÐÀûÀ¸·Î MDPC-23 ¼¼Æ÷´Â ¾Æ±³Áú¿¡ ºÎÂø ģȼºÀ» °®°í ÀÖÀ¸¸ç, ºÎÂø ½Ã¿¡ ÀÎÅױ׸° , ¿Í CD44 ±×¸®°í chondroitin sulfate¿Í °°Àº ºÎÂøºÐÀÚµéÀÌ °ü¿©ÇÑ´Ù. ±×¸®°í TGF-Àº ÀÎÅױ׸° , ±×¸®°í chondroitin sulfate¿Í °°Àº ºÎÂøºÐÀÚÀÇ ¹ßÇöÀ» Áõ°¡½ÃÄ×´Ù.
Odontoblasts are anchorage dependent cells adhering to a substrate via cell adhesive molecules. Receptor ligands such as integrins bind to these proteins and are known to function as signal transduction molecules in a series of critical recognition events of cell-substratum. The aim of this study is to examine the interaction of odontoblast (MDPC-23 cell) with type I Col and the effect of TGF- and TNF- on the expression of cell adhesion molecules. In this study, MDPC-23 cells adhered to type I Col dose-dependently. Immunofluorescence data demonstrated that integrin , and CD44 were expressed on cell surface, and FAK and paxillin were localized in focal adhesion plaques in MDPC-23 cells adhesion to Col. Cytokine TGF- increased the adhesion of MDPC-23 cells to Col and the expression level of integrin , and chondroitin sulfate on MDPC-23 cells. RT-PCR data demonstrated that cytokine TGF- increased the amount of integrin mRNA in MDPC-23 cells. Therefore, MDPC-23 cells adhere to collagen type I Col and expressed a complex pattern of integrins and proteoglycans, including , , chondroitin sulfate and CD44 detected by immunoblotting and immunofluorescence assay. TGF- treatment enhanced the expression of adhesion molecules such as integrin , and chondroitin sulfate.
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Odontoblas;Collagen type I;Cell adhesion
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